Publications

2017:

Javier Ganz, Erez Shor, Shaowei Guo,  Anton Sheinin, Ina Arie, Izhak Michaelevski ,Sandu Pitaru,  Daniel Offen and Shulamit Levenberg
Implantation of 3D Constructs Embedded with Oral Mucosa-Derived Cells Induces Functional Recovery in Rats with Complete Spinal Cord Transection
Front. Neurosci. (2017) doi: 10.3389/fnins.2017.00589

Jonathan Avesar, Dekel Rosenfeld, Marianna Truman-Rosentsvit, Tom Ben-Arye, Yuval Geffen, Moran Bercovici, and Shulamit Levenberg
Rapid phenotypic antimicrobial susceptibility testing using nanoliter arrays
PNAS (2017) doi: 10.1073/pnas.1703736114

Shahar Ben-Shaul, Shira Landau & Shulamit Levenberg
Implanted scaffolds: Pre-ordered vessels halt ischaemia
Nat. Biomed. Eng. (2017) 1, s41551–017 – 0089.

Barak Zohar, Yaron Blinder, David J. Mooney, and Shulamit Levenberg.
Flow-Induced Vascular Network Formation and Maturation in Three-Dimensional Engineered Tissue
ACS Biomater. Sci. Eng (2017). DOI: 10.1021/acsbiomaterials.7b00025

Perry L, Flugelman MY, Levenberg S.
Elderly Patient-Derived Endothelial Cells for Vascularization of Engineered Muscle.
Mol Ther. 2017 Apr 5;25(4):935-948. doi: 10.1016/j.ymthe.2017.02.011

Alina Freiman, Yulia Shandalov, Dekel Rosenfeld, Erez Shor, Dror Ben-David, Shai Meretzki, Shulamit Levenberg and Dana Egozi
You have full text access to this contentEngineering Vascularized Flaps Using Adipose-Derived Microvascular EC and MSC
J Tissue Eng Regen Med (2017). doi: 10.1002/term.2436.

Shira Landau, Ariel A. Szklanny, Giselle C. Yeod, Yulia Shandalov, Elena Kosobrodova, Anthony S. Weiss, Shulamit Levenberg
Tropoelastin coated PLLA-PLGA scaffolds promote vascular network formation
Biomaterials (2017), doi: 10.1016/j.biomaterials.2017.01.015.

Anat Marom, Erez Shor, Shulamit Levenberg and Shy Shoham.
Spontaneous Activity Characteristics of 3D “Optonets”
Front. Neurosci., 10:602. 2017

2016:

Dado-Rosenfeld D, Landau S, Shandalov Y, Raindel N, Shor E, Blinder Y, Vandenburgh H, Mooney D and Levenberg S.
Morphogenesis of 3D vascular network is regulated by tensile forces.
PNAS, 113(12):3215-20. 2016

Egozi D, Shandalov Y, Dado-Rosenfeld D, Freiman A, Ben-Shimol D.,Levenberg S.
Engineered vascularized muscle flap.
JOVE. 107. 2016

Lesman A, Rosenfeld D, Landau S, Levenberg S.
Mechanical regulation of vascular network formation in engineered matrices.
Adv Drug Deliv Rev 96:176-82. 2016

Freiman A, Shandalov Y, Rosenfeld D, Shor E, Segal S, Ben-David D, Meretzki S, Egozi D, Levenberg S.
Adipose-derived endothelial and mesenchymal stem cells enhance vascular network formation on 3D constructs in vitro.
Stem Cell Research & Therapy 7:5. 2016

2015:

Blinder YJ, Freiman A, Raindel N, Mooney DJ, Levenberg S.
Vasculogenic dynamics in 3D engineered tissue constructs.
Sci Rep 5:17840. 2015

Ben-Arye, T., Park S, Shemesh J, Peer D, Levenberg S, Yossifon G.
Dielectrophoretic characterization of cells in a stationary nanoliter droplet array with generated chemical gradients.
Biomed. Microdevices 17, 9996. 2015

Shandalov Y, Egozi D, Freiman A, Dado-Rosenfeld D and Levenberg S
A method for constructing vascularized muscle flap.
Methods 84:70-5. 2015

Dado-Rosenfeld D, Tzchori I, Fine A, Chen-Konak L, Levenberg S
Tensile forces applied on a cell-embedded three-dimensional scaffold can direct early differentiation of embryonic stem cells toward the mesoderm germ layer.
Tissue Eng Part A. 2015

2014:

Anat Weizman, Inbal Michael, Naama Wiesel-Motiuk, Alireza Rezania and Shulamit Levenberg
The effect of endothelial cells on hESC-derived pancreatic progenitors in a 3D environment.
Biomater. Sci. 2014

Shemesh J, Ben Arye T, Avesar J, Kang JH, Fine A, Super M, Meller A, Ingber DE, Levenberg S
Stationary nanoliter droplet array with a substrate of choice for single adherent/nonadherent cell incubation and analysis.
PNAS 2014

Shandalov Y, Egozi D, Koffler J, Dado-Rosenfeld D, Ben-Shimol D, Freiman A, Shor E, Kabala A, Levenberg S
An engineered muscle flap for reconstruction of large soft tissue defects.
PNAS 2014

Avesar J, Ben Arye T, Levenberg S
Frontier microfluidic techniques for short and long-term single cell analysis
Lab On a Chip 2014

Blinder YJ, Mooney DJ, Levenberg S
Engineering approaches for inducing blood vessel formation
Curr Opin Chem Eng 30:8(12) 2014

 

2013:

Shamis Y, Silva EA, Hewitt K J, Brudno, Levenberg S, Mooney DJ, Garlick JA
Fibroblasts Derived from Human Pluripotent Stem Cells Activate Angiogenic Responses In Vitro and In Vivo.
PLOS One 2013

Levenberg S
Guest Editorial: New biomaterials for therapy. Israel Journal of Chemistry
Israel Journal of Chemistry 9-10:607 2013

Ne’eman N, Marbach D, Chen-Konak L, Kaufman-Francis K, Berkovich M, Levenberg S, Lotan N, Sivan S
Multifactor, Sequentially Releasing Scaffolds for Tissue Engineering: Fabrication Using Solvent/Nonsolvent Sintering Technology.
Israel Journal of Chemistry 9-10: 821-828 2013

Dado D. and Levenberg S
Induction of angiogenesis and vasculogenesis using biomaterials
Israel Journal of Chemistry 9-10: 815-820 (2013)

Rozitsky L, Fine A, Dado D, Nussbaum-Ben-Shaul S, Levenberg S, Yossifon G.
Quantifying continuous-flow dielectrophoretic trapping of cells and micro-particles on micro-electrode array
Biomed Microdevices 15(5):859-65 (2013)

Blumenthal J, Cohen-Matsliah SI, Levenberg S
Olfactory bulb-derived cells seeded on 3D scaffolds exhibit neurotrophic factor expression and pro-angiogenic properties
Tissue Engineering Journal Part A 19:2284-91 (2013)

Nseir N, Regev O, Kaully T, Blumenthal J, Levenberg S, Zussman E.
Biodegradable scaffold fabricated of electrospun albumin fibers: mechanical and biological characterization
Tissue Engineering Journal Part C 19(4):257-64. (2013)

2012:

Kaufman-Francis K, Koffler J, Weinberg N, Dor Y, Levenberg S.
Engineered vascular beds provide key signals to pancreatic hormone-producing cells.
PLoS One 2012

Tzezana R, Reznik S, Blumenthal J, Zussman E, Levenberg S.
Regulation of stem cell differentiation by control of retinoic acid gradients in hydrospun 3D scaffold.
Macromol Biosci. 2012

Dado D, Sagi M, Levenberg S, Zemel A.
Mechanical control of stem cell differentiation.
Regen Med. 2012

2011:

Zoldan J, Karagiannis ED, Lee CY, Anderson DG, Langer R, Levenberg S.
The influence of scaffold elasticity on germ layer specification of human embryonic stem cells.
Biomaterials. 2011

Koffler J, Kaufman-Francis K, Shandalov Y, Egozi D, Pavlov DA, Landesberg A, Levenberg S.
Improved vascular organization enhances functional integration of engineered skeletal muscle grafts.
Proc Natl Acad Sci U S A. 2011

Shemesh J, Nir A, Bransky A, Levenberg S.
Coalescence-assisted generation of single nanoliter droplets with predefined composition.
Lab on a Chip. 2011

Lesman A, Koffler J, Atlas R, Blinder YJ, Kam Z, Levenberg S.
Engineering vessel-like networks within multicellular fibrin-based constructs.
Biomaterials. 2011

2010:

Khoury M., Bransky A., Korin N., Chen Konak L., EnikolopovG., Tzchori I. and Levenberg S.
A microfluidic traps system supporting prolonged culture of human embryonic stem cells aggregates.
Biomedical Microdevices. 2010

Shemesh J., Bransky A., Khoury M. and Levenberg S.
Advanced microfluidic droplet manipulation based on piezoelectric actuation.
Biomedical Microdevices. 2010

Levenberg S., Ferreira L., Chen-Konack L., Kraehenbuehl T, and Langer R.
Isolation, differentiation, and characterization of vascular cells derived from human embryonic stem cells.
Nature Protocols. 2010

Michael I., Walton D. and Levenberg S.
Infantile Aphakic Glaucoma: A Proposed Etiologic Role of IL-4 and VEGF.
Journal of Pediatric Ophthalmology. 2010

Lesman A., Gepstein L. And Levenberg S.
Vascularization shaping the heart.
Ann N Y Acad Sci. 2010

2009:

Lesman A., Blinder Y. and Levenberg S.
Modeling of flow-induced shear-stress applied on 3D cellular scaffolds for vascular tissue-engineering applications.
Biotechnology and Bioengineering. 2009

Lumelsky Y., Lalush-Michael I., Levenberg S., and Silverstein M.S.
Degradable, Porous, Emulsion-templated Polyacrylate.
Journal of Polymer Science Part A: Polymer Chemistry 2009

Lesman A., Habib M., Gepstein A., Arbel G., Levenberg S. and Gepstein L.
Transplantation of Tissue-Engineered Human Vascularized Cardiac Muscle.
Tissue Engineering Part A 2009

Dado D, Levenberg S.
Cell-scaffold mechanical interplay within engineered tissue.
Semin Cell Dev Biol. 2009

Kaully T, Kaufman-Francis K, Lesman A, Levenberg S.
Vascularization–the conduit to viable engineered tissues.
Tissue Eng Part B Rev. 2009

Bransky A., Korin N., Khoury M. and Levenberg S.
A Microfluidic Droplet Generator Based on a Piezoelectric Actuator.
Lab on a chip. 2009

Korin N, Bransky A, Khoury M, Dinnar U, Levenberg S.
Design of Well and Groove Microchannel Bioreactors for Cell Culture.
Biotechnology and Bioengineering Journal. 2009

Levy-Mishali M., Zoldan J and Levenberg S.
Effect of Scaffold Stiffness on Myoblast Differentiation.
Tissue Engineering. Part A. 2009

Korin N., Bransky A., Dinnar U. and Levenberg S.
Periodic “Flow-Stop” Perfusion Microchannel Bioreactors for Mammalian and Human Embryonic Stem Cell Long-term Culture.
Biomedical Microdevices. 2009

2008:

Tzezana R. Zussman E., Levenberg S.
A Layered Ultra-Porous Scaffold for Tissue Engineering, created via a Hydrospinning Method.
Tissue Engineering. Part C. 2008

Michael I., Shmoish M., Walton D and Levenberg S.
Interactions Between Trabecular Meshwork Cells and Lens Epithelial Cells – A Possible Mechanism of Infantile Aphakic Glaucoma.
IOVS (Invest Ophthalmol Vis Sci) 2008

Bransky A., Korin N. and Levenberg S.
Experimental and theoretical study of selective protein deposition using focused micro laminar flows.
Biomedical Microdevices. 2008

Lumelsky Y., Zoldan J., Levenberg S. and Silverstein M.S.
Porous Polycaprolactone-Polystyrene Semi-interpenetrating Polymer Networks Synthesized within High Internal Phase Emulsions.
Macromolecules 2008

2007:

Korin N. and Levenberg S.
Engineering human embryonic stem cell differentiation.
Biotechnology and Genetic Engineering Reviews. 2007

Korin N., Bransky A., Dinnar U. and Levenberg S.
A Parametric Study of Human Fibroblasts Culture in a Microchannel Bioreactor.
Lab on a Chip 2007

Korin N., Bransky A., Dinnar U., and Levenberg S.,
A micro-well perfusion bioreactor for human embryonic stem cell
Proc. microTAS 2007

Levenberg S., Zoldan J., Bashevits Y. and Langer R.
Endothelial potential of Human Embryonic Stem Cells.
Blood 2007

Caspi O. and Lesman A., Basevitch Y., Gepstein A., Arbel G., Habib M., Gepstein L., andLevenberg S.
Tissue Engineering of Vascularized Cardiac Muscle from Human Embryonic Stem Cells.
Circulation Research. 2007

2006:

Korin N., Branski A., Dinnar U. and Levenberg S.
The culture of human embryonic stem cells in microchannel perfusion bioreactors.
Proc. SPIE Biomedical Applications of Micro- and Nanoengineering III 2006

2005:

Levenberg S.
Endothelial tissue engineering.
Journal of Vascular Research. 42 suppII: 7 (2005)

Levenberg, S.
Engineering blood vessels from stem cells: recent advances and applications.
Current opinion in Biotechnology. 2005

Levenberg, S., Rouwkema, J., Macdonald, M., Gerfein, E., Kohane, D., Darland D., Marini, R., van Blitterswijk, C.A., Mulligan, R., D’Amore, P. and Langer, R.
Engineering Vascularized Skeletal Muscle Tissue.
Nature Biotechnology. 2005
Cited as a Landmark paper is Nature Biotechnology News and Views.

Levenberg, S., Burdick, J.A., Kraehenbuehl, T., and Langer, R.
Neurotrophin Induced Differentiation of Human Embryonic Stem Cells on Three-Dimensional Polymeric Scaffolds.
Tissue Engineering. 2005

2004:

Levenberg S, Langer R.
Advances in tissue engineering.
Curr Top Dev Biol. 2004

Anderson, D.G., Levenberg, S., and Langer, R.
Nanoliter-scale synthesis of arrayed biomaterials and its application to human embryonic stem cells.
Nature Biotechnology. 2004

Khademhosseini, A., Suh, K.Y., Yang, J.M., Eng, G., Yeh, J., Levenberg, S. and Langer, R.
Layer-by-layer Deposition of Hyaluronic Acid and Poly-L-lysine for Patterned Cell Co-Cultures.
Biomaterials. 2004

2003 and earlier:

Levenberg, S., Huang, N., Erin, L., Rogers, A., Itskovitz-Eldor, J. and Langer, R.
Differentiation of Human Embryonic stem cells on Three Dimensional Polymer Scaffolds.
PNAS. 2003

Levenberg, S., Golub, J. S., Amit, M., Itskovitz-Eldor, J. and Langer, R.
Endothelial Cells Derived From Human Embryonic Stem Cells.
PNAS 2002
Cited in the list of 20 most cited papers on hESC from 1998-2004

Levenberg, S., Yarden, A., Kam, Z. and Geiger, B.
p27 is Involved in N-cadherin-mediated Contact Inhibition of Growth and S-phase Entry.
Oncogene. 18:869-876 (1999)

Levenberg, S., Sadot, E., Goichberg, P. and Geiger, B.
Cadherin – Mediated Transmembrane Interactions.
Cell adhesion and Communication. 6:161-170 (1998)

Kats, B.Z., Levenberg, S., Yamada, K.M., and Geiger, B.
Modulation of Cell-Cell Adherence Junctions by Surface Clustering of the N-Cadherin Cytoplasmic Tail.
Experimental Cell Research. 243:415-424 (1998)

Levenberg, S., Yamada, Katz BZ, Yamada KM, Geiger B.
Long-Range and Selective Autoregulation of Cell-Cell or Cell Matrix Adhesions by Cadherin or Integrin Ligands. J.
Cell Science. 111:347-357 (1998)

Simcha, I., Geiger, B., Yehuda-Levenberg, S., Salomon, D., and Ben-Ze’ev, A.
Suppression of Tumorigenicity by Plakoglobin: An Augmenting Effect of N-cadherin.
J. Cell. Biolog. 1996

Geiger, B., Yehuda-Levenberg, S. and Barshadsky, A.
Molecular Interactions In The Submembrane Plaque of Cell-Cell and Cell-Matrix Adhesions.
Acta Anat. 1995

books:

Lesman A, Gepstein L, Levenberg S.
Cell tri-culture for cardiac vascularization.
Methods Mol Biol. 2014

Zoldan, J. and Levenberg, S.
Culturing and Differentiation of hES cells on three dimensional scaffolds. Methods in Molecular Biology – New Stem Cell Techniques.
Humana Press. In Press

Soukup C., Levenberg S. and Cleaver O.
In vitro differentiation of endothelial cells from human embryonic stem cells. Human Embryonic Stem Cells: A Practical Approach.
Edited by Stephen Sullivan, Chad A Cowan, Kevin Eggan. John Wiley & Sons. NJ. 13, 229-248 (2007)

Malda, J., Baaijens, F., Levenberg, S., Radisic, M., Svalander, P., Woodfield, T. and Vunjak-Novakovic, G.
Cell nutrition. In Textbook on tissue engineering.
Edited by van Blittersvijk, C., Lindahl, A., Thomsen, P., Williams, D., Hubbell, J. and Cancellah, R. Academic Press. Amsterdam. 327-362. (2008)

J. Zoldan and S. Levenberg
Engineering Three-Dimensional Tissue Structures Using Stem Cells. Methods in Enzymology: Stem Cells Volume.
Edited by R. Lanza and I. Kilmanskaya, Elsevier. 381-3420 (2006)

Levenberg, S., Khademhosseini, A., Fuller, J. and Langer, R.
Methods of Human Embryonic Stem Cell Culture. In Culture of Cells for Tissue Engineering.
Editors: Ian Freshney and Gordana Vunjak-Novakovic. John Wiley & Sons. NJ. 61-83. 2006

Levenberg, S., Khademhosseini, A. and Langer, R.
Embryonic Stem Cells in Tissue Engineering. In Handbook of Embryonic Stem Cells.
Editors: Robert Lanza, Doug Melton, James Thomson, John Gearhart, Brigid Hogan, Ron McKay, Roger Pedersen and Mike West. Academic Press. Boston. 737-764. 2006

Levenberg, S., Huang, N. and Langer, R.
Derivation of Endothelial Cells from Human ES Cells. In Human Pluripotent Stem Cells
Editors: Jon S. Odorico, Roger A. Pedersen, and Su-Chun Zhang. BIOS Scientific Publishers Ltd. London. 137-148. 2005